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rabbit anti p-p65 (cell signaling western blot ab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti p-p65 (cell signaling western blot ab
    Rabbit Anti P P65 (Cell Signaling Western Blot Ab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+p-p65+(cell+signaling+western+blot+ab/anti+p38/pm35777852-102-15-16
    Average 90 stars, based on 1 article reviews
    rabbit anti p-p65 (cell signaling western blot ab - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: A druggable redox switch on SHP1 controls macrophage inflammation
    Article Snippet: Lysates were pre-cleared by centrifugation at 21,000 g, 10 min, 4°C, reduced with 4x NuPAGE LDS sample buffer (Invitrogen, #NP0007) containing 5% β-mercaptoethanol, and boiled at 95°C for 10 min. .. Equal amounts of proteins were resolved using NuPAGE 4–12% Bis-Tris 15-well or 26-well gels (Invitrogen, #NP0336BOX or #WG1403BOX), transferred onto PVDF membranes (Thermo Fisher Scientific, iBlot 2 Gel Transfer Device, #IB21001), and immunoblotted with anti-IRAK1 (Cell Signaling, #4504, rabbit mAb; 1:1000 dilution), anti-phospho-IκBα (Ser32/36) (Cell Signaling, #9246; mouse mAb; 1:1000 dilution), anti-IκBα (Cell Signaling, #9242, rabbit pAb; 1:1000 dilution), anti-phospho-NFκB p65 (Ser536) (Cell Signaling, #3033, rabbit mAb; 1:1000 dilution), anti-NFκB p65 (Cell Signaling, #8242, rabbit mAb; 1:1000 dilution), anti-SHP1 (Cell Signaling, #3759, rabbit mAb; 1:1000 dilution), anti-phospho-SHP1 (Tyr536) (Invitrogen, #PA5–36682, rabbit pAb; 1:1000 dilution), anti-phospho-SHP1 (Tyr564) (Cell Signaling, #8849, rabbit mAb; 1:1000 dilution), anti-phospho-SHP1 (Ser591) (Invitrogen, #PA5–143764, rabbit pAb; 1:1000 dilution), anti-STAT3 (Cell Signaling, #4904, rabbit mAb; 1:1000 dilution), anti-phospho-STAT3 (Tyr705) (Cell Signaling, #9145, rabbit mAb; 1:1000 dilution), anti-phospho-STAT3 (Ser727) (Cell Signaling, #9136, mouse mAb; 1:1000 dilution) and anti-β-actin (Cell Signaling, #3700, mouse mAb; 1:3000 dilution) antibodies, diluted in TBS-T (Boston BioProducts, Inc., #IBB-180X) containing 5% BSA, followed by incubation with horseradish peroxidase-conjugated anti-rabbit or anti-mouse secondary antibodies (Promega, #W4011 or #W4021), diluted 1:10000 in TBS-T containing 5% milk, and visualized using ECL western blotting substrates (Pierce ECL Western Blotting Substrate, #32106). ..

    Western Blot:

    Article Title: A druggable redox switch on SHP1 controls macrophage inflammation
    Article Snippet: Lysates were pre-cleared by centrifugation at 21,000 g, 10 min, 4°C, reduced with 4x NuPAGE LDS sample buffer (Invitrogen, #NP0007) containing 5% β-mercaptoethanol, and boiled at 95°C for 10 min. .. Equal amounts of proteins were resolved using NuPAGE 4–12% Bis-Tris 15-well or 26-well gels (Invitrogen, #NP0336BOX or #WG1403BOX), transferred onto PVDF membranes (Thermo Fisher Scientific, iBlot 2 Gel Transfer Device, #IB21001), and immunoblotted with anti-IRAK1 (Cell Signaling, #4504, rabbit mAb; 1:1000 dilution), anti-phospho-IκBα (Ser32/36) (Cell Signaling, #9246; mouse mAb; 1:1000 dilution), anti-IκBα (Cell Signaling, #9242, rabbit pAb; 1:1000 dilution), anti-phospho-NFκB p65 (Ser536) (Cell Signaling, #3033, rabbit mAb; 1:1000 dilution), anti-NFκB p65 (Cell Signaling, #8242, rabbit mAb; 1:1000 dilution), anti-SHP1 (Cell Signaling, #3759, rabbit mAb; 1:1000 dilution), anti-phospho-SHP1 (Tyr536) (Invitrogen, #PA5–36682, rabbit pAb; 1:1000 dilution), anti-phospho-SHP1 (Tyr564) (Cell Signaling, #8849, rabbit mAb; 1:1000 dilution), anti-phospho-SHP1 (Ser591) (Invitrogen, #PA5–143764, rabbit pAb; 1:1000 dilution), anti-STAT3 (Cell Signaling, #4904, rabbit mAb; 1:1000 dilution), anti-phospho-STAT3 (Tyr705) (Cell Signaling, #9145, rabbit mAb; 1:1000 dilution), anti-phospho-STAT3 (Ser727) (Cell Signaling, #9136, mouse mAb; 1:1000 dilution) and anti-β-actin (Cell Signaling, #3700, mouse mAb; 1:3000 dilution) antibodies, diluted in TBS-T (Boston BioProducts, Inc., #IBB-180X) containing 5% BSA, followed by incubation with horseradish peroxidase-conjugated anti-rabbit or anti-mouse secondary antibodies (Promega, #W4011 or #W4021), diluted 1:10000 in TBS-T containing 5% milk, and visualized using ECL western blotting substrates (Pierce ECL Western Blotting Substrate, #32106). ..

    Article Title: Duhuo Jisheng Decoction Mitigates Osteochondral Degeneration in Knee Osteoarthritis through Modulation of Complement C3a/C3AR1-IKKβ-NF-κB Signaling
    Article Snippet: GA (purity ≥98% by high performance liquid 165 chromatography (HPLC), HY-N0764, MedChemExpress, South Brunswick Township, 166 NJ, USA) and the IKKβ inhibitor BMS 345541 (S8044, Selleck Chemicals, Houston, 167 TX, USA) were dissolved in dimethyl sulfoxide (DMSO) at 10 mM stock concentration 168 for mechanistic assays. .. Primary antibodies for immunohistochemistry (IHC), 169 immunofluorescence (IF), and Western Blot (WB) included: anti-COL2 (ab34712, 170 Abcam, Cambridge, UK), anti-MMP13 (ab39012, Abcam, Cambridge, UK), anti-171 NOS2 (YM8628, ImmunoWay, San Jose, CA, USA), anti-C-C motif chemokine ligand 172 2 (CCL2) (YM8646, ImmunoWay, San Jose, CA, USA), anti-interleukin-6 (IL-6) 173 (ab208113, Abcam, Cambridge, UK), anti-C3 (ab11887, Abcam, Cambridge, UK), 174 anti-C3AR1 (ab108129, Abcam, Cambridge, UK), anti-IKKβ (#8943, CST, Danvers, 175 MA, USA), anti-phospho-IKKβ (Ser177/181; #2697, CST, Danvers, MA, USA), anti-176 IκBα (#4814, CST, Danvers, MA, USA), anti-phospho-IκBα (Ser32/36; #9246, CST, 177 Danvers, MA, USA), anti-P65 (#8242, CST, Danvers, MA, USA), anti-phospho-P65 178 (Ser536; #3033, CST, Danvers, MA, USA), and anti-glyceraldehyde-3-phosphate 179 dehydrogenase (GAPDH) (60004-1-Ig, Proteintech, Rosemont, IL, USA). .. Horseradish 180 J urn al Pr e-p roo f peroxidase (HRP)-conjugated secondary antibodies (#7074 and #7076, CST, Danvers, 181 MA, USA) and Alexa Fluor 594-conjugated secondary antibody (A11012, Invitrogen, 182 Carlsbad, CA, USA) were used according to assay requirements.

    Article Title: Metabolic Syndrome Develops Cardia Cancer via Nuclear Factor-E2-related Factor 2-Programmed Death-Ligand 1 Signaling
    Article Snippet: Anti-NRF2 , Abcam , ab137550 , 1:1000 , WB , Rabbit. .. Anti-p-NF-κB p65 , Cell Signaling Technology , 3033S , 1:1000 , WB , Rabbit. .. Anti-PD-1 , Cell Signaling Technology , 84651T , 1:200 , IHC , Rabbit.

    Article Title: SLC6A14-mediated glutamine promotes SYTL4–CXCL8 axis activation to drive gemcitabine resistance and immune evasion in pancreatic cancer
    Article Snippet: Human SLC6A14 shRNA lentiviral particles (cat. no. TL309295V) were purchased from OriGene Technology. .. Antibodies for western blot were as follows: anti-Granuphilin-A (cat. no. sc-374544, 1:1000), anti-NRF2 (cat. no. sc-365949, 1:1000), anti-GPX4 (cat. no. sc-166570, 1:1000), anti-GAPDH (cat. no. sc-365062, 1:1000), anti-OCT3/4 (cat. no. sc-5279, 1:1000), anti-DRP1 (cat. no. sc-101270, 1:1000) and anti-OPA1 (cat. no. sc-393296, 1:1000) antibodies, purchased from Santa Cruz; anti-E-cadherin (cat. no. 610181, 1:1000) and anti-N-cadherin (cat. no. 610920, 1:1000) antibodies, purchased from BD BioScience; anti-EpCAM (cat. no. 2929S, 1:1000), anti-KLF4 (cat. no. 12173S, 1:1000), anti-phospho-mTOR (cat. no. 2974S, 1:1000), anti-mTOR (cat. no. 2983S, 1:1000), anti-p-NF-κB (cat. no. 3033S, 1:1000), anti-NF-κB (cat. no. 8242S, 1:1000), anti-LC3B (cat. no. 2775S, 1:1000) and anti-p-DRP1 (cat. no. 3455S, 1:1000) antibodies, purchased from Cell Signaling Technology; anti-SLC6A14 (cat. no. PA5-87998, 1:1000) antibody, purchased from Thermo Fisher Scientific; anti-RRM1 (cat. no. ab137114, 1:1000), anti-alpha smooth muscle actin (cat. no. ab5694, 1:1000) and anti-Ki-67 (cat. no. ab15580, 1:1000) antibodies, purchased from Abcam; horseradish peroxidase (HRP)-conjugated goat anti-mouse secondary (cat. no. 7076S, 1:1000) and HRP-conjugated goat anti-rabbit secondary (cat. no. 7074S, 1:1000) antibodies, purchased from Cell Signaling Technology. .. Antibodies for immunofluorescent staining were as follows: goat anti-rabbit IgG (H + L) cross-adsorbed secondary antibody, Texas Red-X (cat. no. T-6391, 1:50) and goat anti-mouse IgG (H + L) cross-adsorbed secondary antibody, Alex Fluor 488 (cat. no. A-11001, 1:100), purchased from Invitrogen.

    other:

    Article Title: Lithospermic acid, a novel KLK5 inhibitor, ameliorates rosacea by suppressing the TLR4/NF-κB signaling pathway and rectifying phenylalanine metabolism
    Article Snippet: Primary antibodies used included anti-Toll-like receptor (TLR)4 (Rabbit mAb, Cat. No. #14358, CST), anti-p65 (RelA) (Rabbit mAb, Cat. No. #8242, CST), and anti-phospho-p65 (p-p65) (Ser536) (Rabbit mAb, Cat. No. #3033, CST).

    Article Title: A druggable redox switch on SHP1 controls macrophage inflammation
    Article Snippet: Lysates were pre-cleared by centrifugation at 21,000 g, 10 min, 4°C, reduced with 4x NuPAGE LDS sample buffer (Invitrogen, #NP0007) containing 5% β-mercaptoethanol, and boiled at 95°C for 10 min.

    Immunohistochemistry:

    Article Title: Duhuo Jisheng Decoction Mitigates Osteochondral Degeneration in Knee Osteoarthritis through Modulation of Complement C3a/C3AR1-IKKβ-NF-κB Signaling
    Article Snippet: GA (purity ≥98% by high performance liquid 165 chromatography (HPLC), HY-N0764, MedChemExpress, South Brunswick Township, 166 NJ, USA) and the IKKβ inhibitor BMS 345541 (S8044, Selleck Chemicals, Houston, 167 TX, USA) were dissolved in dimethyl sulfoxide (DMSO) at 10 mM stock concentration 168 for mechanistic assays. .. Primary antibodies for immunohistochemistry (IHC), 169 immunofluorescence (IF), and Western Blot (WB) included: anti-COL2 (ab34712, 170 Abcam, Cambridge, UK), anti-MMP13 (ab39012, Abcam, Cambridge, UK), anti-171 NOS2 (YM8628, ImmunoWay, San Jose, CA, USA), anti-C-C motif chemokine ligand 172 2 (CCL2) (YM8646, ImmunoWay, San Jose, CA, USA), anti-interleukin-6 (IL-6) 173 (ab208113, Abcam, Cambridge, UK), anti-C3 (ab11887, Abcam, Cambridge, UK), 174 anti-C3AR1 (ab108129, Abcam, Cambridge, UK), anti-IKKβ (#8943, CST, Danvers, 175 MA, USA), anti-phospho-IKKβ (Ser177/181; #2697, CST, Danvers, MA, USA), anti-176 IκBα (#4814, CST, Danvers, MA, USA), anti-phospho-IκBα (Ser32/36; #9246, CST, 177 Danvers, MA, USA), anti-P65 (#8242, CST, Danvers, MA, USA), anti-phospho-P65 178 (Ser536; #3033, CST, Danvers, MA, USA), and anti-glyceraldehyde-3-phosphate 179 dehydrogenase (GAPDH) (60004-1-Ig, Proteintech, Rosemont, IL, USA). .. Horseradish 180 J urn al Pr e-p roo f peroxidase (HRP)-conjugated secondary antibodies (#7074 and #7076, CST, Danvers, 181 MA, USA) and Alexa Fluor 594-conjugated secondary antibody (A11012, Invitrogen, 182 Carlsbad, CA, USA) were used according to assay requirements.

    Immunofluorescence:

    Article Title: Duhuo Jisheng Decoction Mitigates Osteochondral Degeneration in Knee Osteoarthritis through Modulation of Complement C3a/C3AR1-IKKβ-NF-κB Signaling
    Article Snippet: GA (purity ≥98% by high performance liquid 165 chromatography (HPLC), HY-N0764, MedChemExpress, South Brunswick Township, 166 NJ, USA) and the IKKβ inhibitor BMS 345541 (S8044, Selleck Chemicals, Houston, 167 TX, USA) were dissolved in dimethyl sulfoxide (DMSO) at 10 mM stock concentration 168 for mechanistic assays. .. Primary antibodies for immunohistochemistry (IHC), 169 immunofluorescence (IF), and Western Blot (WB) included: anti-COL2 (ab34712, 170 Abcam, Cambridge, UK), anti-MMP13 (ab39012, Abcam, Cambridge, UK), anti-171 NOS2 (YM8628, ImmunoWay, San Jose, CA, USA), anti-C-C motif chemokine ligand 172 2 (CCL2) (YM8646, ImmunoWay, San Jose, CA, USA), anti-interleukin-6 (IL-6) 173 (ab208113, Abcam, Cambridge, UK), anti-C3 (ab11887, Abcam, Cambridge, UK), 174 anti-C3AR1 (ab108129, Abcam, Cambridge, UK), anti-IKKβ (#8943, CST, Danvers, 175 MA, USA), anti-phospho-IKKβ (Ser177/181; #2697, CST, Danvers, MA, USA), anti-176 IκBα (#4814, CST, Danvers, MA, USA), anti-phospho-IκBα (Ser32/36; #9246, CST, 177 Danvers, MA, USA), anti-P65 (#8242, CST, Danvers, MA, USA), anti-phospho-P65 178 (Ser536; #3033, CST, Danvers, MA, USA), and anti-glyceraldehyde-3-phosphate 179 dehydrogenase (GAPDH) (60004-1-Ig, Proteintech, Rosemont, IL, USA). .. Horseradish 180 J urn al Pr e-p roo f peroxidase (HRP)-conjugated secondary antibodies (#7074 and #7076, CST, Danvers, 181 MA, USA) and Alexa Fluor 594-conjugated secondary antibody (A11012, Invitrogen, 182 Carlsbad, CA, USA) were used according to assay requirements.



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    rabbit anti p-p65 (cell signaling western blot ab  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc rabbit anti p-p65 (cell signaling western blot ab
    Rabbit Anti P P65 (Cell Signaling Western Blot Ab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+p-p65+(cell+signaling+western+blot+ab/anti+p38/pm35777852-102-15-16
    Average 90 stars, based on 1 article reviews
    rabbit anti p-p65 (cell signaling western blot ab - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

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